New Method for Early Cancer Diagnosis – A real-time analysis of the early diagnosis of cancer markers

The NSFC funded Key Program project “A real-time analysis of the early diagnosis of cancer markers”, undertaken by Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CAS), recently received recognition from an expert team for assessment. Experts pointed out that the research was of great value for the fast ascertain of cancer markers and the early warning and diagnosis. The research team was headed by Academician Wang Erkang, CAS and Prof. Kong Jilie, Fudan University.

Starting from January, 2004, the group has put their utmost to the research and attained cheerful results. By means of capillary electrophoresis (CE) with electro-chemiluminescence (ECL) detection, surface plasma resonance (SPR), laser induced fluorescence (LIF), spectrum technology, protein chips, gene chip sensors, etc., a real-time, sensitive and specific online detection of some cancer markers was established.

After their successful commercialization of the capillary electrophoresis electrochemical analyzer as an independent intellectual property, the group further developed new ECL technologies and ECL probes, working out a new method for simple, sensitive detection of the activity of prolidase (PLD) in plasma and serum, marker of some cancers such as liver cancer and mammary cancer. The combination of various fast, high sensitive and selective analytical methods was realized in the newly developed analysis and micro total analysis system, owing to which microchips for detecting various proteins and genes were brought into being. The application of these new methods to the detection of clinical cases contributed to the construction of clinical substance and analysis parameter databases. The dynamics of the influence of drugs on the activity of PLD in red blood cells (RBC) was investigated and, in accord, functions of different drugs in increasing or decreasing the risk of cancer were revealed. A new approach was developed for the detection of the activity of aspartate aminotransferase and alanine aminotransferase, and the toxic impact of ethanol on hepatoma cells was also studied. By the adoption of the electrochemical method together with a variety of spectrum technologies, detailed study on the interaction between the anti-cancer drug Mitoxantrone, Calf Thymus DNA, and Cytochrome C was conducted, providing a theoretical basis for oriented synthesis of drugs.

With regard to the protein chip or gene chip sensor, the researchers also developed immunochips in crossing finger shape and silicon chips of integrated three-electrode to detect serum markers of liver fibrosis. Based on the study of selective reversible assembly of antibody protein characteristic of electrochemical surface switch, the microfluidic chip with switching functions was developed for controlled protein separation. The research progress gained so far was of great importance to clinical applications such as the fast analysis of body/tissue fluids of patients, the construction of databases of analysis parameter for clinical substances, and the fast detection of cancer markers for early warning and diagnosis.

The project won the research group 4 prizes and 16 patents, and produced 87 papers, of which, 50 were published by key academic journals abroad (SCI impact factor > 3), and 38 conference papers.

Source: National Natural Science Foundation of China (NSFC), http://www.nsfc.gov.cn/

Advertisements

~ by vascoteixeira on September 2, 2008.

 
%d bloggers like this: